microbiologists improve taste of beer
Overview of experimental methods used in this study. (A) Lab-scale determination of the CO2 overpressure inhibition of acetate ester production (isoamyl acetate/alcohol ~AATase activity). (B) Mating and breeding segregants (offspring) with a range of CO2-inhibition of AATase activity, followed by selection of a pool of segregants with a superior acetate ester production profile. (C) Whole-genome sequence analysis of the superior pool and bioinformatic analysis to identify QTLs responsible for the trait. (D) Schematic representation of (Bulk) RHA as used to identify the causative gene in QTL 2. (E) Graphical overview of the 2 strategies and different plasmids used for CRISPR/Cas9 mediated MDS3 allele exchange. Credit: Applied and Environmental Microbiology (2022). DOI: 10.1128/aem.00814-22

Belgian investigators have improved the flavor of contemporary beer by identifying and engineering a gene that is responsible for much of the flavor of beer and some other alcoholic drinks. The research appears in Applied and Environmental Microbiology.

For centuries, beer was brewed in open, horizontal vats. But in the 1970s, the industry switched to using large, closed vessels, which are much easier to fill, empty, and clean, enabling brewing of larger volumes and reducing costs. However, these modern methods produced inferior quality beer, due to insufficient flavor production.

During fermentation, yeast converts 50 percent of the sugar in the mash to ethanol, and the other 50 percent to carbon dioxide. The problem: the carbon dioxide pressurizes these closed vessels, dampening flavor.

Johan Thevelein, Ph.D., an emeritus professor of Molecular Cell Biology at Katholieke Universiteit, and his team had pioneered technology for identifying genes responsible for commercially important traits in yeast. They applied this technology to identify the gene(s) responsible for flavor in beer, by screening large numbers of yeast strains to evaluate which did the best job of preserving flavor under pressure.

They focused on a gene for a banana-like flavor “because it is one of the most important flavors present in beer, as well as in other alcoholic drinks,” said Thevelein, who is also founder of NovelYeast, which collaborates with other companies in industrial biotechnology.

“To our surprise, we identified a single mutation in the MDS3 gene, which codes for a regulator apparently involved in production of isoamyl acetate, the source of the banana-like flavor that was responsible for most of the pressure tolerance in this specific yeast strain,” said Thevelein.

Thevelein and coworkers then used CRISPR/Cas9, a gene editing technology, to engineer this mutation in other brewing strains, which similarly improved their tolerance of carbon dioxide pressure, enabling full flavor. “That demonstrated the scientific relevance of our findings, and their commercial potential,” said Thevelein.

“The mutation is the first insight into understanding the mechanism by which high carbon dioxide pressure may compromise beer flavor production,” said Thevelein, who noted that the MDS3 protein is likely a component of an important regulatory pathway that may play a role in carbon dioxide inhibition of banana flavor production, adding, “how it does that is not clear.”

The technology has also been successful in identifying genetic elements important for rose flavor production by yeast in alcoholic drinks, as well as other commercially important traits, such as glycerol production and thermotolerance. More information: Ben Souffriau et al, Polygenic Analysis of Tolerance to Carbon Dioxide Inhibition of Isoamyl Acetate “Banana” Flavor Production in Yeast Reveals MDS3 as Major Causative Gene, Applied and Environmental Microbiology (2022). DOI: 10.1128/aem.00814-22 Journal information: Applied and Environmental Microbiology

Provided by American Society for Microbiology Citation: Microbiologists improve taste of beer (2022, October 4) retrieved 4 October 2022 from https://phys.org/news/2022-10-microbiologists-beer.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

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